Fig. 4.
125I-VT-1 binding to neutral glycolipid extracts from human PMNs, GMVECs, and monocytes.
Glycolipids were extracted and separated as described in “Materials and methods.” Binding of 125I-VT-1 was visualized, using a phosphor-imager. (A) Glycolipid extract of 30 million PMNs of 1 representative donor. (B) Neutral glycolipid extraction of TNF-α–treated GMVECs. (C) Glycolipid extract of monocytes stimulated with LPS (1 ng/mL). (D) Standard mixture of neutral glycolipids, 2 μg of each glycolipid. Standard of neutral glycolipids was stained with orcinol. (Gb3, globotriaosylceramide; Gb4, globotetraosylceramide; and Gb5, Forssman pentasaccharide).
