Fig. 7.
Measurement of protein synthesis by incorporation of3H-leucine in newly synthesized proteins.
(A) Nonstimulated GMVECs incubated with VT-1 loaded PMNs showed during 24-hour incubation a reduction of protein synthesis. (B) GMVECs prestimulated for 24 hours with TNF-α (10 ng/mL) showed strong inhibition during incubation with VT-1 alone or after incubation with VT-1 preloaded PMNs. Similar results were obtained from 5 different donors for GMVECs. Results are expressed as ± SEM. Statistical analysis was performed, using unpaired Studentt test. P values smaller than .05 were considered to be significant. * P < 0.05 as compared to nonstimulated GMVECs (A) and TNF-α–prestimulated GMVECs (B).
