Expression of Flt-1 in wounded monolayers of endothelial cells.

HUVEC monolayers were partially denuded and formaldehyde fixed at 0 (A, B), 5 (C, D), 10 (E, F), 15 (G, H), or 20 hours (I, J). (B, D, F, H, J) Cells were incubated with a rabbit-specific antibody against Flt-1 and a Cy3-conjugated goat antirabbit secondary antibody (green). Control cells (A, C, E, G, I) were incubated with the secondary antibody alone. All preparations were counterstained with DAPI to localize cell nuclei. Similar results were obtained in 2 independent experiments.