Endothelial denudation and PMA enhance flt-1promoter-dependent transcription.

HUVEC were transiently transfected with the flt-1 (−1195 to +284) promoter luciferase construct pFlt-1–luc or the luciferase reporter plasmid containing 3 consensus binding sites for Egr-1 p3EGR–Luc. Cell monolayers were mechanically denuded or treated with PMA (50 ng/mL) for 8 hours. Luciferase activities were normalized for transfection efficiency to the renilla luciferase activities. Data shown are means ± SE of 3 independent experiments with 3 independent wells in each.