RT-PCR analysis of gp91-phox transcripts in total cell and nuclear RNA preparations.

RT-PCR products of gp91-phox transcripts from the normal (Nl) or CGD patient's EBV-transformed B-cell lines, cultured with or without IFN-γ, as indicated (+IFN). Upper panel: Representative agarose electrophoresis gels, stained with ethidium bromide, of RT-PCR products from the RNA sources indicated below the image. Primers corresponded to cDNAs encoding gp91-phox or β-actin, as indicated in the left margin; size markers are indicated in the right margin. Lower panel: Graph representing compiled data from 3 experiments. Band intensity was quantitated by digital photography and computer analysis with Molecular Analyst software. Data are expressed as percentage expression relative to gp91-phox transcript levels in normal total cellular RNA, adjusted for β-actin signals; columns indicate means and error bars show SDs.