The effect of IFN-γ on splicing of nuclear CYBBtranscripts in CGD and normal B-cell lines.

RT-PCR products of CYBB nuclear mRNA amplification performed with a forward primer in the first exon and alternative reverse primers in the fifth exon or the first intron (as diagramed in Figure 4) detect, respectively, spliced (S) and unspliced (U) transcripts from nuclei prepared from normal (Nl) or CGD B-cell lines, incubated with (+IFN) or without IFN-γ. Upper panel: Representative ethidium bromide-stained agarose gel of RT-PCR products from the RNA sources indicated below the image; size markers are shown in the right margin. Lower panel: Graph representing compiled data from 3 experiments. Band intensity was quantitated by digital photography and computer analysis with Molecular Analyst software. Data are expressed as the ratio of spliced to unspliced products; columns represent means and error bars show SDs.