FACS analysis of PB at early time points after transplantation.

Sheep that had been transplanted in utero with human HSC alone or in combination with autologous stromal cells were killed at early time points of 3, 6, and 9 weeks after transplant and their PB was collected. FACS analysis for the presence of the human-specific marker CD45 was then performed to evaluate whether stromal cells could enable the early entry of donor human cells within the periphery of animals transplanted in utero. Values represent the mean ± 1 SEM.