Fig. 2.
CHX and bisindolylmaleimide (Bis) down-regulate c-FLIPL expression.
DC were incubated for 12 hours in medium alone or in the presence of either CHX (10 μg/mL) or Bis III (20 μmol/L). Whole-cell extracts were electrophoresed and probed with anti-FLIPL mAb (NF6), anti-Bcl-2 mAb, anti-Bcl-xL rabbit polyclonal antibody (Ab), or anti-Bax rabbit polyclonal Ab, followed by an appropriate second Ab. Two representative experiments of 10 performed on samples from different blood donors are shown.
