Fig. 5.
![Fig. 5. Binding of PfEMP1 domains to heparin and heparin fragments. / (A) [3H] Heparin binding to 4 μg of DBL-1 (—•—), CIDR (—♦—), and DBL-4 (—○—) in an in-solution assay was performed with increasing concentrations of [3H] heparin and protein together with bound polysaccharide was recovered by membrane filtration as described in “Materials and methods.” (B) Size defined [3H] heparin fragments were allowed to bind to the proteins under the same conditions as in A: DBL-1 (—•—), CIDR (—♦—), and DBL-4 (—○—).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/11/10.1182_blood.v95.11.3594/5/bloo01121005x.jpeg?Expires=1735851612&Signature=m35295kSaTmvV4qV6sU6aSzXJYbZfwcSiIZh9ceyYyXoXe8OmrTmIIprfKfKxN5gVhOwdUKQO9sVd~MKqIgIL2sxjz-xmsyEmzmPm5DCcurgO0nKXSleRlHs6WgnBFaKt4T08b2ib~~nVXTNJvoIi3oltx4bNrxBxbrwS2gbLK4wkS6vf~td3bVAEyKwKG28VGkY4MFVOSinBeScikrU6-M4Ed7trKvGpY9~f4fieeATcVNJ-BOGMyftcQdmQxTzLibHI5lOpObC5SVRYJiQiF3I3MTSir1pb79gu7BAEWhRF4wrgwXQYXL1vQzsP1fULQaFZGnqQ59GcW5xnakhPg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Binding of PfEMP1 domains to heparin and heparin fragments.
(A) [3H] Heparin binding to 4 μg of DBL-1 (—•—), CIDR (—♦—), and DBL-4 (—○—) in an in-solution assay was performed with increasing concentrations of [3H] heparin and protein together with bound polysaccharide was recovered by membrane filtration as described in “Materials and methods.” (B) Size defined [3H] heparin fragments were allowed to bind to the proteins under the same conditions as in A: DBL-1 (—•—), CIDR (—♦—), and DBL-4 (—○—).
