Formation of 5′HS 3, 5′HS 4, and minor associated sites in mice with the 5′HS 4 region deleted.

The restriction digest is with SphI, and the probe is probe 4 from Figure 1. M denotes the marker lane; the DNaseI concentration is 0 in the first lane; and increases as shown by the shaded triangle. The portion of the film to the left of the marker shows an assay done on wild-type mice, and below this is the restriction map of expected bands. The portion of the autoradiograph to the right of the marker shows an assay done on mice with 5′HS 4 deleted, and below this is the restriction map of expected bands.