HbF reactivation and synthesis.

(A) HbF reactivation in minibulk HPC erythroid cultures supplemented or not with KL (100 ng/mL) ± NaB (0.5 mmol/L) or ± TSA (10 ng/mL). Clonogenetic parameters are shown in top panels. On the bottom, Hb synthesis is shown as F cells and γ-chain content percentage. Mean ± SEM values from 3 separate experiments. Variance analysis was performed to evaluate the difference between groups and expressed as Bonferroni P values. *P < .05 and ***P < .001 when compared with the indicated group. (B) HbF synthesis, evaluated in terms of F cells and γ-chain content, in pooled sibling BFU-E colonies grown in unilineage erythroid culture supplemented or not with KL ± NaB or ± TSA. Mean ± SEM values from 3 separate experiments. *P < .05 and ***P < .001 when compared with the indicated group.