Fig. 4.
Determination of kinetic parameters of the binding of affinity-purified anti-FVIII IgG from IVIg to the C2 fragment of FVIII.
F(ab′)2 fragments of anti-FVIII IgG were covalently coupled to the sensor chip. Real-time binding was studied for C2 in HBS-CaCl2 at the concentrations indicated. At the time indicated by the arrow, the ligand solution was replaced by HBS-CaCl2 alone, and dissociation curves were recorded. Ordinate represents the binding response as measured by resonance units (RU).
