FKLF-2 mRNA.

(A) FKLF-2 mRNA expression by Northern blotting. Each lane contains 2 μg poly(A)+RNA from adult human tissues. The RNA was blotted with a specific FKLF-2 probe. A distinct band at about 135 kb was detected in the bone marrow and the heart and skeletal muscle. Another major band between 4.4 and 7.5 kb seemed to be a cross hybridization to 28S ribosomal RNA because of its size. (B) FKFL-2 mRNA expression in various cell lines by RT-PCR. Three bands in each picture show the results of amplification in different cycles; ie, from left to right, 34, 32, and 30 cycles for FKLF-2, and 22, 20, and 18 cycles for 28S rRNA. (C) FKLF-2 mRNA expression in K562 and HEL cells before (ui) and after (i) induction by Northern blottings. Two micrograms poly(A)+RNA was blotted with probes indicated. (D) FKLF-2 mRNA expression in MEL cells before and after induction (Northern blotting). Four micrograms poly(A)+RNA was blotted with probes indicated. The position of the FKLF-2 band is approximately 6 kb.