Trans-activation of ɛ and γ and β globin gene promoters by FKLF-2.

Reporter constructs containing a luciferase gene driven by HS2 and ε, γ, or β gene promoter were transfected into K562 cells with or without pSG5/FKLF-2. Luciferase activities were corrected by protein concentrations, and expressed as relative percentages of luciferase activity of pHS2γLuc that were not cotransfected by pSG5/FKLF-2 (100%). Protein concentration was used to correct the transfection efficiency, because in our preliminary experiments we could not rule out that FKLF-2 does not activate the SV-40 promoter/enhancer of the pSVβ-Gal control plasmid.3 Data are expressed as mean (columns) ± SD (error bars) derived from multiple transfections using 2 different plasmid sets.