Effect of truncation deletions of γ gene promoter on FKLF-2 activity.

Reporter constructs containing a luciferase gene driven by various γ gene promoters depicted in Figure 4 were tested in K562 cells. Luciferase activities were expressed as relative percentages of luciferase activity of pγLuc that were not cotransfected by pSG5/FKLF-2 (100%). Fold increases (expressed as mean ± SD) of luciferase activities by FKLF-2 compared with those in the absence of the transactivator are shown below.