Effect of FKLF-2 on various erythroid promoters.

(A) Reporter constructs containing a luciferase gene driven by either GATA-1 (GATA), porphobilinogen deaminase (PBGD), ferrochelatase (FC), glycophorin B (GPB), or 5-aminolevulinate synthase (ALAS) promoter were cotransfected with or without pSG5/FKLF-2. (B) Fold increase of luciferase activities by FKLF-2 from the reporter constructs used in this experiment. Numbers above the promoters are base pair distances from the cap site (γ, GPB, FC, PBGD, and ALAS) or from the end of exon 1 (GATA), and they indicate the upstream ends of the promoter sequences cloned, and the positions of the CACCC and the GC-rich sequences (solid rectangles and open ellipses, respectively).