Inhibition of tyrosine phosphorylation in STI571-treated Ba/F3.p210 cells.

(A) Ba/F3.p210 cells were incubated in the presence of increasing concentrations of STI571 (0.01 to 100 μmol/L) for 2 hours. Immunoblotting of the lysates from these cells was performed with anti-pTyr (upper panel), anti-ABL (3F12) (middle panel), and anti-SHP2 as a control for protein loading (lower panel). (B) Ba/F3.p210 cells were incubated in the presence of increasing concentrations of STI571 (0.01 to 10 μmol/L) for 24 hours. Immunoblotting of the lysates from these cells was performed as in (A). (C) K562 cells were incubated in the presence of increasing concentrations of STI571 (0.01 to 10 μmol/L) for 2 hours. Immunoblotting of the lysates from these cells was performed as in (A).