BCR/ABL protein expression in STI571-resistant Ba/F3.p210 cells.

Nonresistant Ba/F3.p210 cells cultured in the presence of DMSO were treated for 20 minutes with 1 mmol/L AEBSF or water, as vehicle. STI571-resistant Ba/F3.p210 cells were cultured in the continuous presence of 1 μmol/L STI571 and then treated with 1 mmol/L AEBSF for 20 minutes. STI571-resistant Ba/F3.p210 cells were deprived of STI571 for 24 and 48 hours, respectively, and then treated with 1 mmol/L AEBSF for 20 minutes. Immunoblotting of the lysates from these cells was performed with anti-ABL (upper left-hand panel), anti-BCR (middle left-hand panel), and anti-SHP2 as a control for loading (lower left-hand panel), and anti-pTyr (upper right-hand panel).