Inhibition of cellular tyrosine phosphorylation in nonresistant Ba/F3.p210 cells and STI571-resistant Ba/F3.p210 cells by STI571.

Nonresistant Ba/F3.p210 cells were treated for 2 hours with DMSO, 1 μmol/L STI571, or 10 μmol/L STI571. STI571-resistant Ba/F3.p210 cells were treated for 2 hours with DMSO, 1 μmol/L STI571, or 10 μmol/L STI571, following a 2-hour STI571 deprivation. Immunoblotting of the lysates from these cells was performed with anti-pTyr (left-hand panel), anti-ABL (upper right-hand panel), and anti-SHP2 as a control for loading (lower right-hand panel).