Fig. 3.
Fig. 3. GM-CSF binding activity of wild-type receptors. / The COS cells were either transfected with GMRα alone or cotransfected with plasmids encoding GMRα and plasmids encoding GMRβ at the indicated ratios. 125I-labeled GM-CSF binding of the transfected cells was determined at 48 hours after transfection by Scatchard analysis. (A) α/β = 2:1, high-affinity kd = 229.9 pmol/L, low-affinity kd = 9.01nmol/L. (B) α/β = 1:6, kd = 216.9 pmol/L. (C) GMRα alone, kd = 9.10 nmol/L.

GM-CSF binding activity of wild-type receptors.

The COS cells were either transfected with GMRα alone or cotransfected with plasmids encoding GMRα and plasmids encoding GMRβ at the indicated ratios. 125I-labeled GM-CSF binding of the transfected cells was determined at 48 hours after transfection by Scatchard analysis. (A) α/β = 2:1, high-affinity kd = 229.9 pmol/L, low-affinity kd = 9.01nmol/L. (B) α/β = 1:6, kd = 216.9 pmol/L. (C) GMRα alone, kd = 9.10 nmol/L.

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