Fig. 4.
Effect of PP1 on LTB4-induced ERK-2 phosphorylation.
Cells were preincubated at 37°C for 5 minutes with the indicated concentration of PP1 and stimulated with LTB4 (100 nmol/L), and the reaction was stopped at the indicated times by the addition of immunoprecipitation buffer. Cell homogenates were separated by SDS-PAGE, and the presence of dual phosphorylated ERK-2 was detected by Western blot analysis. Representative blots obtained from 3 separate preparations are shown.
