Fig. 5.
![Fig. 5. Identification of a Ca++-dependent and Ca++-independent pathway of [3H]AA release. / Cells were preincubated for 5 minutes at 37°C in the presence (○) or absence (•) of 1 mmol/L Ca++ and 1 mmol/L Mg++. Cells were then stimulated with the indicated concentration of LTB4. Data represent the mean ± SEM of 3 to 4 independent experiments and show (A) the maximal increase in [Ca++]c and (B) the release of [3H]AA release at 60 seconds, where an asterisk indicates that the stimulation of the LTB4-induced Ca++-independent release was significant (P < .05) compared to controls.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/11/10.1182_blood.v95.11.3541/6/bloo01111005x.jpeg?Expires=1735591999&Signature=SnMw1u3T5PEUrPKTx5M6gWDiRD-0LLw~galUhSkvy64gYxktYrAU0LiS6OeaVH3BHYHOfGVqL9lgGtambMNXQXZZSG~vDWEmfgln0td2NtxV6ZkfzsueNHJq2NIysq6sMfiu~MSgJEtt9y6qTBwlJ7K64fa~U0Ing37wirUqnJn9nIHFWkiUGn03XN7ndRUPQ6Lfnc71MWIiq2uZvh3KvUSeezL-BIxLW569viVoWqqXd8MlT~EI26-MKT1KxLQILBk~esmgBNBa-nGRqpEj~C7~k3Uk~nPb7302RggnxsvNZUXdIGb6-dZoyuCjZck5eqPGslxJJdxtRxR-0v15~g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Identification of a Ca++-dependent and Ca++-independent pathway of [3H]AA release.
Cells were preincubated for 5 minutes at 37°C in the presence (○) or absence (•) of 1 mmol/L Ca++ and 1 mmol/L Mg++. Cells were then stimulated with the indicated concentration of LTB4. Data represent the mean ± SEM of 3 to 4 independent experiments and show (A) the maximal increase in [Ca++]c and (B) the release of [3H]AA release at 60 seconds, where an asterisk indicates that the stimulation of the LTB4-induced Ca++-independent release was significant (P < .05) compared to controls.
