Fig. 6.
![Fig. 6. Effect of PP1 on LTB4-induced [3H]AA release and superoxide generation. / Cells were preincubated for 5 minutes at 37°C in the presence (○) or absence (•) of 1 mmol/L Ca++/1 mmol/L Mg2+ and the indicated concentrations of PP1. Data represent the mean ± SEM of 3 to 4 independent experiments. (A) Following stimulation with LTB4 (100 nmol/L), the release of [3H]AA at 60 seconds was determined. (B) Following stimulation with LTB4(100 nmol/L), the maximal rate of superoxide generation measured as relative light units (RLU) was determined. An asterisk indicates significant inhibition of LTB4-induced response by the indicated concentration of inhibitor compared with controls. P < .05.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/11/10.1182_blood.v95.11.3541/6/bloo01111006x.jpeg?Expires=1735590852&Signature=UHKdj1Rx4qRNpWO5xw2VhEYLdynlJM~4TFMeTXFTMJsTA9OaNw3Tc5Kww5uigzikLTSwlM2ldIFOOnwHzML0ZcJmfGJ0bCQm3OMTE07tXSK0qj9g8h82vW160yQWzZpa2JsRWXzdHJk0Q81ogtq1v-pmP3-Um2lJo2VUJf0fHPjocQ9wQVeKRYtkuGESo3R3ZXOWu8vO6gw9qFleHbwdiMtGq9VeSlSsk62kcWU5SyVs2L7OfokUAHimd--YJBUuTCtXej1f0LlFKGUeXHUCIiLZ2z8GtwHQ~-xYtAPOH0tX7jw6QyuOM8TNfM6xTctroTGtz9-w1NnhgWOOWtP4qQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of PP1 on LTB4-induced [3H]AA release and superoxide generation.
Cells were preincubated for 5 minutes at 37°C in the presence (○) or absence (•) of 1 mmol/L Ca++/1 mmol/L Mg2+ and the indicated concentrations of PP1. Data represent the mean ± SEM of 3 to 4 independent experiments. (A) Following stimulation with LTB4 (100 nmol/L), the release of [3H]AA at 60 seconds was determined. (B) Following stimulation with LTB4(100 nmol/L), the maximal rate of superoxide generation measured as relative light units (RLU) was determined. An asterisk indicates significant inhibition of LTB4-induced response by the indicated concentration of inhibitor compared with controls. P < .05.
