![Fig. 3. Effect of PI 3-kinase inhibitors on platelet aggregation and secretion of 5-hydroxytryptamine (5-HT). / Platelets were isolated as described in “Materials and methods” and resuspended at 2 to 4 × 108/mL in a modified Tyrode's buffer. Platelets were treated with LY294002 (LY, i) or wortmannin (Wt, ii) for 3 minutes at 37°C before stimulation. All experiments were performed in a Born aggregometer. (A) and (B) An increase in optical density (OD) represents shape change that precedes the decrease, due to aggregation. The traces are representative of at least 10 experiments. (C) [3H]5-HT secretion was analyzed as described in “Material and methods,” the concentrations of LY294002 and Wt were 50 μmol/L and 100 nmol/L, respectively. Platelets were incubated with collagen for 2 minutes. Results are shown as the mean ± SEM of 3 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/11/10.1182_blood.v95.11.3429/6/bloo01122003x.jpeg?Expires=1735824173&Signature=jaeX694GXCQnNSObmc-bed9l5-THw15wys7ZiyEIw9a2UtfCk4BbEQYVwyeqMMBXRdGxSE~gbdGN9FxCbIvFmQVla6S3m-GF4mkIp92LMfAOKRYUvRGe0Sp18GY6ljN0mXHffeUsE-jDVZTKLw1xsRTLUkw3tj53dXlGMkMsTQAkGv6ajaPqTtGR-yjnmIelEhJgdWiaxkOWrcM7UtWg1kcTn3ejdLemWAtRDXujAs0vlFgt7SeXMmpzLHf~AujauJm0Z9gz~vkVHvDADSPjSc-HvPgvIWXAXtv0wQT-dohSBz9ikzrpjFhygjWCdh6U-RMLAeulozr-D2s-irEEpw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of PI 3-kinase inhibitors on platelet aggregation and secretion of 5-hydroxytryptamine (5-HT).
Platelets were isolated as described in “Materials and methods” and resuspended at 2 to 4 × 108/mL in a modified Tyrode's buffer. Platelets were treated with LY294002 (LY, i) or wortmannin (Wt, ii) for 3 minutes at 37°C before stimulation. All experiments were performed in a Born aggregometer. (A) and (B) An increase in optical density (OD) represents shape change that precedes the decrease, due to aggregation. The traces are representative of at least 10 experiments. (C) [3H]5-HT secretion was analyzed as described in “Material and methods,” the concentrations of LY294002 and Wt were 50 μmol/L and 100 nmol/L, respectively. Platelets were incubated with collagen for 2 minutes. Results are shown as the mean ± SEM of 3 experiments.
