Fig. 1.
Angiogenesis in the CAM is inhibited by endostatin dependent on its heparin-binding ability.
Ten-day chick embryos were incubated with filter disks saturated with buffer alone, or with FGF-2 with or without wild-type and mutated endostatins (ES). The effect of the additions on CAM angiogenesis was analyzed after 3 days of incubation, by excising the filter and microscopical examination. The R158/270A mutant lacks heparin-binding ability and the H134A, D136A mutant lacks Zn2+-binding ability.
