Fig. 2.
Tubular morphogenesis of murine brain endothelial cells is supported by endostatin.
IBE cells were cultured between 2 layers of collagen gels, in Ham's F12, 0.25 mg/mL BSA. The cultures were incubated in the absence of additions (control) or in the presence of 1 μg/mL of endostatin and 5 ng/mL of FGF-2 alone or in combination for 24 or 48 hours. The cultures were analyzed by light microscopy and photographed (×20 objective).