Coimmunoprecipitation of TEL2.

(A) Coimmunoprecipitation of TEL2 with HATEL2. Human fibroblast cells (293T) were transiently transfected with empty vector, HAT2, T2, or HAT2 + T2, and lysates were immunoprecipitated with 12CA5. Immunocomplexes were separated by SDS-PAGE, Western blotted, and incubated with α-T2. Lysates from cells transfected with the indicated constructs were also Western blotted with α-T2 (right 3 lanes). MW standards are indicated on the left. (B) Coimmunoprecipitation of TEL2 and TEL1. Lysates of Hela cells transiently transfected with the indicated expression constructs were incubated with either α-T2 (left 3 lanes) or α-T1 antibodies (right 3 lanes). Immunoprecipitates were separated by SDS-PAGE, Western blotted, and incubated with the 12CA5 aby. MW standards are indicated on the right. (C) 293T cells were transiently transfected with the constructs indicated above each lane and lysates were immunoprecipitated with either α-T2 or α-T1. Immunoprecipitates were separated by SDS-PAGE, Western blotted, and incubated with 12CA5. MW standards are shown on the left.