Reduced numbers of DC present in the lymphoid tissues of flt3L−/− mice.

DC were enriched from the spleen, thymus, and LN (n = 6/mouse) by first treating the tissues with collagenase followed by centrifugation over Nycodenz to enrich for low-density cells. Cells were incubated with antibodies against CD11c and CD8α. A representative example of 3 to 5 separate experiments is shown in which tissues from 3 to 8 mice were pooled (see Table 2).