FACS analysis.

(A) Bone marrow, spleen, and peripheral blood cells from 3 individual mice (no. 1-3) at 14 weeks of age were analyzed for Gr-1 (Ly-6G) and Mac-1 (CD11b) staining. (B) Cells from 1 mouse (no. 1) from the RA⁺ and RA⁻ group were tested for markers for B cells (B220/IgM) and T cells (CD4/CD8). (C) Time course analysis. Percent Mac-1⁺/Gr-1⁺ cells in bone marrow and spleen were determined by FACS analysis every 2 weeks. The values represent the average of 3 to 4 mice ± SD.