Impaired spontaneous apoptosis in RA⁻granulocytes.

Spleen cells from a representative RA⁺ or RA⁻mouse (15 weeks old) were cultured overnight and stained with PE-labeled antibodies to Gr-1, B220, and CD3, and then subjected to TUNEL assay. The X axis (FITC) represents DNA fragmentation and the Y axis cell surface markers. The number below the FACS profile represents percent apoptotic cells of 3 mice ± SD.