Effect of ferriporphyrins on ferritin expression.
Human umbilical vein endothelial cell monolayers were treated with medium 199 alone (first lanes and bar) or equimolar (10 μmol/L), various ferriporphyrins, including hematin (second lanes and bar), heme arginate (third lanes and bar), iron deuteroporphyrin IX,2,4-bis-sulfonate (fourth lanes and bar), iron coproporphyrin III (fifth lanes and bar), iron deuteroporphyrin IX,2,4-bis-glycol (sixth lanes and bar), or iron deuteroporphyrin IX (seventh lanes and bar), in media 199 for 60 minutes followed by additional incubations with ferriporphyrin free medium. (A) Northern blot analysis of mRNA for heavy-chain (H) and light-chain (L) ferritin at 4 hours after treatment of endothelial cells. Upper Panel: After transfer and hybridization, the H-ferritin and L-ferritin mRNAs are recognized by 32P-labeled cDNA probes. Lower Panel: Equal quantities of 28S rRNA were shown in the ethidium bromide–stained agarose gel. (B) Ferritin content at 16 hours after treatment of endothelium was measured for the same groups as in (A). Results are expressed as nanograms of ferritin per milligram of endothelial cell protein and represent mean ± SE of at least 3 experiments performed in duplicate. P < .004 heme arginate (third bar) versus control (first bar).
