Kinetics of lipid peroxidation of LDL catalyzed by hematin or heme arginate.

The LDL (200 μg/mL protein) was exposed to hematin (5 μmol/L) (closed squares) or heme arginate (5 μmol/L) (closed circles) in the presence of H₂O₂ (75 μmol/L), hematin alone (5 μmol/L) (open squares), heme arginate alone (5 μmol/L) (open circles), and H₂O₂ alone (75 μmol/L) (open triangles). Closed triangles represent native LDL alone. The lipid peroxidation was monitored spectrophotometrically at 234 nm at 37 °C for 120 minutes to assess the formation of conjugated dienes.