VPS33B and VPS16B form a distinct and small complex. (A) Gel filtration chromatography analysis of recombinant copurified His-VPS33B/TS-VPS16B complex. Fractions were analyzed by immunoblotting with antibodies against VPS33B and VPS16B. (B) Gel filtration chromatography analysis of cytosolic extract obtained from imMKCLs. Fractions were analyzed by immunoblotting with antibodies against VPS33B, VPS16B, and VPS11. The column was calibrated with blue dextran and standard proteins of known hydrodynamic radius.