Figure 4.
LPL/WM features of LPD in MYD88L265Pmice. (A) Flow cytometric analysis of lymphocyte subpopulations in LNs from aged AIDCre (n = 7) and MYD88WT (n = 7) control mice and MYD88L265P (n = 11) mice with LPD. Graphs depict the mean ± SD. P values were calculated by using Welch’s t test. (B) Serum protein electrophoresis of aged AIDCre (n = 2) and MYD88WT (n = 2) control mice and of MYD88L265P (n = 9) mice with LPD. Note increased abundance of diffuse gamma globulins (black arrowheads) in MYD88L265P mice compared with controls. A positive (Pos.) control containing a sharp band of gamma globulins (M spike) is shown for comparison. (C) Serum IgM concentrations assessed using multiplex immunoassay in aged AIDCre (n = 7), MYD88WT (n = 7), and MYD88L265P (n = 23) mice. Orange points represent DLBCL animals in the MYD88L265P cohort. Graphs depict the median ± 25th to 75th percentile. P values were calculated by using the Mann-Whitney U test (see also supplemental Figure 2C). (D) Peripheral blood smears demonstrating rouleaux formation of red blood cells in representative MYD88L265P (n = 3) mice with LPD. (E) Serum IL-6 concentrations assessed by multiplex immunoassay in aged AIDCre (n = 8), MYD88WT (n = 8), and MYD88L265P (n = 24) mice. Orange points represent DLBCL animals in the MYD88L265P cohort; the dotted line indicates the assay sensitivity threshold. Graphs depict the median ± 25th to 75th percentile. P value was calculated by using Fisher’s exact test. P value for the analysis with DLBCL samples omitted is .0491. (F) Serum CXCL13 concentrations assessed by multiplex immunoassay in aged AIDCre (n = 7), MYD88WT (n = 6), and MYD88L265P mice with (n = 7) or without (n = 15) macroscopic liver and/or lung changes. Orange points represent DLBCL animals in the MYD88L265P cohort. Graphs depict the median ± 25th to 75th percentile. P values were calculated by using the Mann-Whitney U test. P values for the analysis with DLBCL samples omitted are .0424, .0381, and .0139 for comparison of MYD88L265P mice with macroscopic liver and/or lung changes with AIDCre, MYD88WT, and MYD88L265P mice without macroscopic liver and/or lung changes, respectively (see also supplemental Figure 2A). Alter., alterations.

LPL/WM features of LPD in MYD88L265Pmice. (A) Flow cytometric analysis of lymphocyte subpopulations in LNs from aged AIDCre (n = 7) and MYD88WT (n = 7) control mice and MYD88L265P (n = 11) mice with LPD. Graphs depict the mean ± SD. P values were calculated by using Welch’s t test. (B) Serum protein electrophoresis of aged AIDCre (n = 2) and MYD88WT (n = 2) control mice and of MYD88L265P (n = 9) mice with LPD. Note increased abundance of diffuse gamma globulins (black arrowheads) in MYD88L265P mice compared with controls. A positive (Pos.) control containing a sharp band of gamma globulins (M spike) is shown for comparison. (C) Serum IgM concentrations assessed using multiplex immunoassay in aged AIDCre (n = 7), MYD88WT (n = 7), and MYD88L265P (n = 23) mice. Orange points represent DLBCL animals in the MYD88L265P cohort. Graphs depict the median ± 25th to 75th percentile. P values were calculated by using the Mann-Whitney U test (see also supplemental Figure 2C). (D) Peripheral blood smears demonstrating rouleaux formation of red blood cells in representative MYD88L265P (n = 3) mice with LPD. (E) Serum IL-6 concentrations assessed by multiplex immunoassay in aged AIDCre (n = 8), MYD88WT (n = 8), and MYD88L265P (n = 24) mice. Orange points represent DLBCL animals in the MYD88L265P cohort; the dotted line indicates the assay sensitivity threshold. Graphs depict the median ± 25th to 75th percentile. P value was calculated by using Fisher’s exact test. P value for the analysis with DLBCL samples omitted is .0491. (F) Serum CXCL13 concentrations assessed by multiplex immunoassay in aged AIDCre (n = 7), MYD88WT (n = 6), and MYD88L265P mice with (n = 7) or without (n = 15) macroscopic liver and/or lung changes. Orange points represent DLBCL animals in the MYD88L265P cohort. Graphs depict the median ± 25th to 75th percentile. P values were calculated by using the Mann-Whitney U test. P values for the analysis with DLBCL samples omitted are .0424, .0381, and .0139 for comparison of MYD88L265P mice with macroscopic liver and/or lung changes with AIDCre, MYD88WT, and MYD88L265P mice without macroscopic liver and/or lung changes, respectively (see also supplemental Figure 2A). Alter., alterations.

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