Effects of murine and human PF4 on circulating NDP levels following LPS exposure in WT and cxcl4^−/−mice. (A) WT and cxcl4^−/− mice received LPS (35 mg/kg, IP). Plasma samples were obtained at the indicated time points. Mean plasma cfDNA levels are shown as ± 1 standard deviation (SD). N = 7-14 mice per arm. P values are indicated comparing WT and cxcl4^−/− mice using a Mann-Whitney U test. (B) The same as panel A, except for MPO levels at 3 to 8 hours post-LPS. (C) Histone levels comparing western blot intensity to that of the positive control band. N = 9-10 mice per arm. P values are indicated comparing WT and cxcl4^−/− mice by Mann-Whitney U test. (D) LPS studies comparing WT and cxcl4^−/− mice following implantation of osmotic pumps containing PBS alone or 80 µg of mPF4. Mean ± 1 SD are shown N = 5-8 mice per arm. (E) The same as panel D, except following a single dose of hPF4 (330 µg, IV). N = 5-8 mice per arm. (F) LPS studies comparing plasma levels of MPO-cfDNA complex in cxcl4^−/− mice that received tail vein injections containing PBS alone or 330 µg of hPF4. N = 4-5 mice per arm. P values are indicated comparing WT and cxcl4^−/− mice by Mann-Whitney U test.