DG-KKO treatment in WT, cxcl4^−/−, and hPF4⁺mice undergoing LPS endotoxemia. Mice were injection with LPS (35 mg/kg, IP). Thirty minutes later, they received tail vein injections containing vehicle alone or 5 mg/kg of DG-KKO or DG-TRA isotype control or DG-KKO plus DNase I (20 mg/kg). Six hours following LPS injection, a subset of mice was euthanized and IVC blood samples were collected. (A) Relative to baseline (dashed gray line), 6-hour time point platelet counts. Mean ± 1 SD shown. (B-E) The same as panel A, but for cfDNA concentration, MPO-cfDNA complex fold change relative to negative control, MCP-1 levels as measured by fold change in western blot bandwidth relative to GAPDH control, and MSS. N = 3-10 in each arm. Comparative statistical analysis was performed with a Kruskal-Wallis 1-way ANOVA. (F) Animal survival results for the LPS studies in the cxcl4^−/− mice and hPF4⁺ mice. Results were analyzed with the log-rank test. n = 5 animals per arm.