DG-KKO treatment in cxcl4^−/−and hPF4⁺mice undergoing CLP injury. All the mice underwent CLP procedure. Immediately following surgery, a subset of mice received an intradermal dose of the antibiotic ceftriaxone (100 mg/kg). Mice also were divided by therapeutic intervention, receiving either vehicle only or 5 mg/kg of TRA, DG-TRA, KKO, or DG-KKO. After 24 hours, platelet counts were quantified. After 48 hours, bacterial CFUs, plasma NDP levels, and survival were measured. Mean ± 1 SD shown. (A) Relative platelet counts 24 hours after CLP injury measured as in Figure 4A. (B) CFUs as measured in the peripheral blood and liver homogenates of animals 48 hours following CLP. Black bars indicate animals that underwent sham surgery. (C-D) The same as Figure 4B-C, respectively, but 48 hours after CLP injury. (A-D) N = 6-10 animals per arm. Statistical analysis performed with a Kruskal-Wallis 1-way ANOVA. (E) Plasma MCP-1 levels in hPF4⁺ mice, measured as in Figure 4D. n = 5. Analysis with a Kruskal-Wallis 1-way ANOVA. (F) Animal survival results for the CLP studies. N = 10. Statistical analysis performed with a log-rank (Mantel-Cox) test, showing a significant increase in survival in hPF4⁺ mice treated with DG-KKO compared with those treated with vehicle alone, KKO, or DG-TRA with and without antibiotics (P < .0001).