WES of T2ΔT3, ΔT2T3, and ΔT2ΔT3 mice. (A) Variant allele frequency plot of mutations revealed by WES of paired tail and BM samples from T2ΔT3, ΔT2T3, and ΔT2ΔT3 mice. Amplification of the Tet3 wild-type allele in BM from ΔT2T3 mice (n = 2; #47 and #60) is highlighted with yellow arrows as shown in Figure 5. (B) Validation of Tet3 mutations identified in WES by Sanger sequencing. (C) Position of Tet3 mutations revealed by exome sequencing in samples #47 (R894X) and #60 (R941X) are shown in the Tet3 gene.