Early and late divergent evolution and N-gly acquirement. (A) Representative of patient 5. N-gly sites are acquired in site-specific precursor cells, explaining the different N-gly sites we observe in the first and third relapse events taken from distinct anatomical sites (Table 2). (B) Representative of patient 6. Here, the N-gly site was acquired in a shared precursor cell before divergence of tumor populations to distinct anatomical sites. G.L, germline. Letters in cells represent the N-gly site amino acid sequences.