Gene signatures in patients after allogeneic HSCT with and without acute GVHD and in individuals who did not receive a transplant. (A) Isolation of iNKT cells. Representative plot showing the results of the purification procedure for all sequencing experiments. (B) Concatenated t-distributed stochastic neighbor embedding (tSNE) plot of single-cell messenger RNA-sequencing of iNKT cells from 1 healthy individual who did not receive a transplant (green), 1 patient after HSCT without GVHD (blue), and 1 patient after HSCT with onset of gastrointestinal GVHD (dark red). A heatmap shows selected differential gene expression (log₁₀ scale of unique molecular identifier [UMI] counts) across the 3 cell clusters detected in the tSNE plots, and violin plots show the normalized distribution of expression of genes between the 3 individuals (bottom right). APC, antigen-presenting cell; FITC, fluorescein isothiocyanate.