American Society of Hematology

Figure 2.

$Short-term kinetic assessment of primary iNKT cells with a targeted scRNA-seq approach. (A) Sequencing of 4000 iNKT cells targeting RNA expression of 312 T-cell–associated genes from 2 healthy donors. The cells from each donor were experimentally divided into 2 fractions: primary cells that received no in vitro activation and cells that were activated in vitro with CD3/CD28 Dynabeads overnight. The data have been dimensionally reduced and displayed as a tSNE plot. (B-D) Combined expression of (B) genes associated with an NK-cell phenotype, (C) genes associated with memory T cells (Tcmem), and (D) distribution of gene expression of immunologically relevant effector molecules across the clusters.$

Short-term kinetic assessment of primary iNKT cells with a targeted scRNA-seq approach. (A) Sequencing of 4000 iNKT cells targeting RNA expression of 312 T-cell–associated genes from 2 healthy donors. The cells from each donor were experimentally divided into 2 fractions: primary cells that received no in vitro activation and cells that were activated in vitro with CD3/CD28 Dynabeads overnight. The data have been dimensionally reduced and displayed as a tSNE plot. (B-D) Combined expression of (B) genes associated with an NK-cell phenotype, (C) genes associated with memory T cells (Tcmem), and (D) distribution of gene expression of immunologically relevant effector molecules across the clusters.

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