CGD mouse neutrophils had increased LTB4 production and elevated calcium influx compared with WT, and oxidant scavengers did not increase LTB4 in WT neutrophils. (A) Mouse BM neutrophils (4 × 10⁶/mL) were stimulated for various times with zymosan (MOI = 2 zymosan: 1 PMN) in RPMI 1640 (0.4 mM Ca²⁺; n ≥ 3). *P < .05; ****P < .0001), by Student t test. (B) Mouse BM neutrophils (4 × 10⁶/mL) were pretreated with 200 U/mL SOD and 200 U/mL catalase for 10 minutes and then stimulated for 1 hour with zymosan (MOI = 2) in RPMI 1640 (0.4 mM Ca²⁺) in the presence of 200 U/mL SOD and 200 U/mL catalase. n = 4 per group from 2 separate experiments. *P < .05, by paired t test. NS, not significant. (C-D) SOCE in neutrophils from WT and CGD mice was measured by flow cytometry. Mouse BM neutrophils (1 × 10⁶/mL) loaded with 3 µM indo-1 were stimulated with (C) Zymosan (100 µg/mL; MOI ≈ 10) with the indicated amounts of extracellular calcium. (D) Quantification of SOCE (area under the curve [AUC]). Mean ± standard error of the mean from 2 to 4 independent experiments. *P < .05; **P < .01, by Student t test. (E) Mouse BM neutrophils (4 × 10⁶/mL) were stimulated for 1 hour with zymosan (MOI = 2) in Hanks’ balanced salt solution with the indicated amounts of extracellular calcium. LTB4 levels in culture supernatant was measured by ELISA. n ≥ 3 per group from 3 separate experiments. Data are means ± SEM. *P < .01; ***P < .001, by Student t test. MOI, multiplicity of infection.