Dysregulated genes involved in thrombosis, HIF target genes, and inflammation in PV patients compared with controls by RNA-seq. Dysregulated thrombosis genes in PV granulocytes (A) and platelets (D). HIF-regulated genes in PV granulocytes (B) and platelets (E). Inflammatory genes in PV granulocytes (C) and platelets (F). Black dots represent genes that do not have significantly different expression levels compared with controls. Green dots are genes with log₂ fold change >1 but not adjusted P (Adjp) < .05. Blue dots are genes with adjusted P < .05 but not log₂ fold change >1. Genes labeled with red dots are differentially expressed genes with adjusted P < .05 and log₂ fold change >1. F3, SELP, SERPINE1, VEGFA, and SLC2A1 were selected to validate expression levels by qRT-PCR (see Figure 5).