Assessment of GVHD. (A) Mean bodyweight in the first 4 weeks of life. Shown is the mean ± standard deviation over time. No experimental group was found by ANOVA to have a lower mean bodyweight than uninjected Balb/c controls at any point. (B) A GVHD phenotype score ranging from 0 to 10 was calculated on the basis of the presence of hunched posture, ruffled fur, fur loss, desquamation, and diarrhea with scores lower than 3 consistent with no GVHD. No animals developed GVHD. (C) Histology of the liver, skin, lungs, and small bowel at 3 weeks of age. Slides were stained with hematoxylin and eosin. Images were collected at 20× magnification. No features of GVHD were detected on histology.