Group 1 and group 2 donors have different TCRγ chains and similar TCRδ chains. (A) Vδ1⁺ (light red, light blue) and Vδ2⁺ (dark red, dark blue) T-cell proportions in total lymphocytes of group 1 (N = 10; red) and group 2 (N = 10; blue). (B) Vγ9Vδ1⁺ and Vγ9Vδ2⁺ T-cell proportions within γδ T cells of group 1 (N = 10; red) and group 2 (N = 10; blue). (C) Frequency of Vδ1 (light red, light blue) and Vδ2 (dark red, dark blue) usage in γδ T cells of group 1 (N = 10; red) and group 2 (N = 10; blue). (D) Frequency of Jδ1 in γδ T cells of group 1 (G1; N = 10; red) and group 2 (G2; N = 10; blue). (E) Kullback-Leibler sequence logos showing residue preferences for the top 20 CDR3δ2 sequences. Due to length variations, the analyses considered the first 10 residues for each CDR3δ2. Data are for representative group 1 and group 2 individuals. Black arrows denote position 5 in the CDR3δ2 sequences. Each logo consists of amino acid stacks, 1 for each position in the sequence. The stack height indicates the extent of sequence conservation; the residue height indicates its relative frequency. Residue colors correspond to biochemical properties: acidic (red), basic (blue), hydrophobic (black), polar (green). Logos were generated using WebLogo.²⁸  (F) Frequency of Vγ9 usage in group 1 (G1; N = 10; red) and group 2 (G2; N = 10; blue). (G) Frequency of Jγ usage in group 1 (N = 13; red) and group 2 (N = 12; blue). (H) Proportions of CDR3γ of different length in group 1 (N = 13; red circles) and group 2 (N = 12; blue diamonds) donors’ TCRγ repertoires. Mean values and standard errors of the mean are given. (I) Proportions of CDR3 Jγ2-Vγ9 among CDR3 Jγ2 in group 1 (G1; N = 13; red) and group 2 (G2; N = 12; blue) donors’ TCRγ repertoires. Statistical significance between groups was assessed using the Student t test (*P = .02; **P = .003; ***P ≤ .0006). ns, not significant.