In responding to EBV, γδ T cells preferentially use the JγP segment. Two hundred thousand γδ T cells were isolated from PBMCs after stimulation with Akata, an EBV-transformed B cell line. (A) Vδ1⁺ (light red, light blue) and Vδ2⁺ T-cell proportions (dark red, dark blue) within EBV-stimulated lymphocytes of group 1 (N = 10; red) and group 2 (N = 10; blue). (B) Vγ9Vδ1⁺ and Vγ9Vδ2⁺ T-cell proportions within EBV-stimulated γδ T cells of group 1 (N = 10; red) and group 2 (N = 10; blue). (C) Frequency of Vδ1 (light red, light blue) and Vδ2 (dark red, dark blue) usage in EBV-stimulated γδ T cells of group 1 (N = 10; red) and group 2 (N = 10; blue). (D) Frequency of Jγ usage by EBV-stimulated γδ T cells of group 1 (N = 10; red) and group 2 (N = 10; blue). (E) Proportions of CDR3γ of different length in group 1 (N = 10; red circles) and group 2 (N = 10; blue diamonds) donors’ TCRγ repertoires. Means and standard errors of the mean are given. Statistical significance was assessed using the Student t test (*P = .02; ****P ≤ .0001).