Figure 4.
Loss of MNT impedes normal B lymphoid development. (A-B) Deletion of floxed Mnt by Rag1Cre provokes lymphopenia in mice lacking Eμ-Myc transgene. Quantification of B lymphoid subpopulations from bone marrow (A) and spleen (B) of 4-week-old WT (light green), Rag1Cre (green), and Mntfl/flRag1Cre (light blue) mice. Supplemental Figure 5A exemplifies sorting strategy. n indicates number of mice. Mean ± SD; **P ≤ .01; ***P ≤ .001; ****P ≤ .0001. (C-D) Activation of CreERT2 by tamoxifen also provokes B lymphopenia in mice lacking Eμ-Myc transgene. Quantification of indicated B lymphoid populations from bone marrow (C) and spleen (D). Young (6-week-old) WT (light green), CreERT2 (gray), and Mntfl/flCreERT2 (lavender) mice were treated for 3 days with tamoxifen by oral gavage and analyzed 4 weeks later. n indicates number of mice. Bar graphs show mean ± SD; *P ≤ .05; **P ≤ .01.

Loss of MNT impedes normal B lymphoid development. (A-B) Deletion of floxed Mnt by Rag1Cre provokes lymphopenia in mice lacking Eμ-Myc transgene. Quantification of B lymphoid subpopulations from bone marrow (A) and spleen (B) of 4-week-old WT (light green), Rag1Cre (green), and Mntfl/flRag1Cre (light blue) mice. Supplemental Figure 5A exemplifies sorting strategy. n indicates number of mice. Mean ± SD; **P ≤ .01; ***P ≤ .001; ****P ≤ .0001. (C-D) Activation of CreERT2 by tamoxifen also provokes B lymphopenia in mice lacking Eμ-Myc transgene. Quantification of indicated B lymphoid populations from bone marrow (C) and spleen (D). Young (6-week-old) WT (light green), CreERT2 (gray), and Mntfl/flCreERT2 (lavender) mice were treated for 3 days with tamoxifen by oral gavage and analyzed 4 weeks later. n indicates number of mice. Bar graphs show mean ± SD; *P ≤ .05; **P ≤ .01.

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