Clonal dynamics (II). (A) Change in VAFs according to the number of mutated genes at baseline (n = 464). The highest absolute difference in VAF is indicated for each individual. Vertical lines indicate means for each subgroup. (B) Forest plot for the risk of acquiring an additional mutation over time for individuals carrying CH, according to the presence of mutations in DNMT3A (n = 254), TET2 (n = 119), or ASXL1 (n = 25). Circles indicate the relative risk for each gene as compared with other mutational spectra at baseline. Horizontal lines indicate 95% CIs. P values are from Fisher’s exact test. (C) Prevalence of CH at baseline (blue) and follow-up (red) according to anemia status at baseline and follow-up (n = 943). (D) Change in VAFs according to anemia status at baseline and follow-up (n = 943). The highest absolute difference in VAF is indicated for each individual. Vertical lines indicate mean differences for each subgroup. For panels C and D, individuals were grouped as never having anemia (n = 485), newly developing anemia (n = 27), having stable anemia over time (n = 207), or having anemia at baseline that is corrected at follow-up (n = 224).