Upregulation of Cebpα and Cebpδ in Myc^V394DAML cells contributes to the partial differentiation phenotype. (A) Representative upregulated genes in Myc^V394-AML cells as identified from RNA sequencing are presented in a heatmap. (B) Myc^V394D-AML cells were collected at the indicated times after 4-OHT treatment as described in Figure 6G-H. The expression of Cebpα and Cebpδ was examined by qRT-PCR. (C) The expression of Cebpα and Cebpδ was examined by qRT-PCR in MSCV-, Myc- and Myc^V394D-transduced HSPCs on day 4 after transduction. (D-E) The binding of Myc, Myc^V394D, and Miz1 to the promoters of the Cebpα or Cebpδ genes was examined in AML cells by ChIP/quantitative PCR (qPCR) assay. (F-G) H3K9Me3 and H3K9Ac status of the promoters of Cebpα or Cebpδ genes were examined by ChIP/qPCR assay. (H-J) Cebpα or Cebpδ or both were knocked down in Myc^V394D AML cells by short hairpin RNA (shRNA) specific for Cebpα (shCα) or Cebpδ (shCδ) (H). The third replating CFC (I) and response to DNR or Ara-C treatment (J) of the gene knockdown cells were compared with scrambled shRNA (scr)-transduced Myc^V394D AML cells. Myc AML cells were used as controls. (K-M) Cebpα or Cebpδ or both were overexpressed in Myc AML cells by viral transduction (K). The third replating CFC (L) and response to DNR or Ara-C treatment (M) of the gene overexpressed cells were compared with empty vector (EV)-transduced Myc-AML cells. **P < .01, ##P < .01, and #P < .05, compared with corresponding Myc groups in panel J and corresponding EV groups in panel M. $$P < .01 and $P < .05, compared with corresponding scr groups. D, distal promoto; P, proximal promoter.