FOXP3⁺Hel-Δ3B mediates different gene transcription and pathway enrichment in CD4⁺and CD8⁺eTregs compared with FOXP3⁺Hel-FL. All comparisons in this figure use FOXP3⁺Hel-FL eTregs as the baseline for comparison, either CD4⁺ or CD8⁺ as indicated. (A) Volcano plots depicting gene changes. Within the volcano plots, genes were colored if they had an FDR ≤0.1. Blue denotes downregulation, and red represents upregulation in the indicated comparison. The 2 vertical lines represent logFC= −2 and logFC = 2. The horizontal line presents –log10(0.05). (B) Summary of normalized enrichment scores (NES) of KEGG pathways with P ≤ .05 that were enriched compared with two eTreg cell strains after GSEA. Blue bars are pathways that are more downregulated in the Hel-Δ3B–expressing cell line, and red bars are pathways that are more upregulated in the Hel-Δ3B cell line, compared with the HEL-FL–expressing cell line.